Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: D4F prophylaxis enables redox and energy homeostasis while preventing inflammation during hypoxia exposure.

doi: 10.1016/j.biopha.2020.111083

Figure Lengend Snippet: Fig. 5. a. Hypoxia responsive signaling cascade associated with apolipoproteins. A STRING [88] based network with colored spheres showing query proteins; medium con fidence (0.400); 20 interactors in second shell showing the links between master regulatory protein (HIF-1a); antioxidants (SOD); inflam matory cytokines (CRP) and metabolic proteins (apolipoproteins). b. Representative immu noblots and bar-graphs depicting pixel in tensities of these immunoblots in HIF-1a and MnSOD in lungs and CRP, Apo-A1 and Apo-B in plasma. HIF-1a pixel intensities in N-2241.368 ± 162.04, ND-3985.723 ± 266.038, H-8679.09 ± 309.545, HD-9264.693 ± 394.014. MnSOD pixel intensities in N-26374.56 ± 754, ND-31417.26 ± 841, H-34801.78 ± 632, HD-39799.67 ± 432. Pixel intensities of CRP in N-990.134 ± 144.87, ND-8519.572 ± 358.33, H-17471.86 ± 511.998, HD-9972.995 ± 655.713. Apo-A1 pixel intensities in N-35004.76 ± 1058.93, ND-40377.78 ± 1264, H-29687.65 ± 1199.14, HD-30482.96 ± 983. Apo-B pixel intensities in N-22390.02 ± 244, ND-23844.02 ± 739.99, H-30615 ± 855.62, HD-24528.61 ± 730.55. All results arepresented as Mean ± SEM in AU (arbitrary units). Significance represented as * (p-value<0.05). Data from three separate bio logical replicates.Equal loading control was whole gel stained with Coomassie blue (Fig. S1; Supplementary information).

Article Snippet: The following primary antibodies were used for immunoblotting: HIF-1a (Cat. # NB100105, Novus Biologicals, USA); SOD (Cat # CSB-PA022398KA01HU; Cusabio, USA); CRP (Cat # ab5086; Abcam, USA); Apo-A1 (Cat. # sc-30089, Santa Cruz, UK) and Apo-B (Cat # CSB-PA318254; Cusabio; USA).

Techniques: Western Blot, Clinical Proteomics, Control, Staining

Journal: Animal Cells and Systems

Article Title: Epinephrine as a potential driver of oral lichen planus pathogenesis

doi: 10.1080/19768354.2025.2588914

Figure Lengend Snippet: High level of epinephrine induces intracellular oxidative stress in oral keratinocytes. (A) HOK-16B cells treated with PBS or epinephrine for 24 h were subjected to CellROX staining. Nuclear DAPI signal is shown in blue (left). The relative CellROX signal intensities are shown (right). Each symbol means an individual cell. (B) Immunoblot analysis of SOD2 and SESN2 in HOK-16B cells treated with epinephrine for 24 h. β ACTIN was used as loading control. (C) Relative band intensities are shown. (D) Relative mRNA expression of TNFα, IL6, and IL8 in HOK-16B cells treated with epinephrine for 3 h. ** P < 0.01; *** P < 0.001. n.s , not significant.

Article Snippet: After blocking with 5% non-fat dried milk in Tris-buffered saline with TweenTM 20 (TBST) (10 mM Tris, pH 8.0, 150 mM NaCl and 0.5% Tween 20) for 30 min, membranes were incubated with antibodies against phospho STAT3 (1:1000, Cell signaling, Danvers, MA, USA, cat no. 9145), total STAT3 (1:1000, Cell signaling, cat no. 8768), β ACTIN (1:5000, Sigma Aldrich, cat no. A5316), phospho ERK (1:1000, Thermo Fisher Scientific, Waltham, MA, USA, cat no. MA5-15174), total ERK (1:1000, Cell signaling, cat no. 4695), phospho AKT (1:1000, Cell signaling, cat no. 4058), total AKT (1:1000, Cell signaling, cat no. 9272), BCL2 (1:1000, Bioworld Technology, China, cat no. BS1511), SOD2 (1:1000, Cusabio, Houston, TX, USA, cat no. CSB-PA022398LA01HU), and SESN2 (1:1000, Abcam, Cambridge, UK, cat no. ab178518) overnight at 4°C.

Techniques: Staining, Western Blot, Control, Expressing